Bitechnology : Principles and Processes Biology Quiz for NEET

No. of Questions in Quiz	28 with Explanation
Time for each Questions	60 Seconds
Attempts Allowed	Unlimited
Available	Always
Pass Percentage	70 %

In any case you found any kind of error or mistakes in this quiz then let us know in our comment box below, and also share your feedback on this quiz.

#1. A selectable marker is used to

Mark a gene on a chromosome for isolation using restriction enzyme

Help in eliminating the non-transformants, so that the transformants can be regenerated

Identify the gene for a desired trait in an alien organism

Select a suitable vector for transformation in a specific crop

Selectable markers help in identification and elimination of non-transformants whilst permitting selective growth of transformants.

#2. An enzyme catalysing the removal of nucleotides from ends of DNA is

Protease

DNA ligase

Endonuclease

Exonuclease

Restriction enzymes belong to a larger class of enzymes called Nucleases.

Nucleases are of two different kinds :Exonucleases which remove nucleotides from the end of a DNA molecule, Endonucleases, which break internal phosphodiester bonds at palindromic sites that are highly specific

DNA ligase helps in joining DNA fragments

#3. The two antibiotic resistance genes on vector pBR322 are for

Tetracycline and Kanamycin

Ampicillin and Tetracycline

Ampicillin and Chloramphenicol

Chloramphenicol and Tetracycline

#4. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

BOD incubator

Sludge digester

Industrial oven

Bioreactor

#5. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with

Isopropanol

Chilled ethanol

Methanol at room temperature

Chilled chloroform

During the isolation of desired gene, chilled ethanol is used for the precipitation of DNA.

#6. Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

The enzyme cuts DNA molecule at identified position within the DNA.

The enzyme binds DNA at specific sites and cuts only one of the two strands.

The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbone.

#7. The correct order of steps in Polymerase Chain Reaction (PCR) is

Extension, Denaturation, Annealing

Annealing, Extension, Denaturation

Denaturation, Annealing, Extension

Denaturation, Extension, Annealing

This technique is used for making multiple copies of gene of interest in vitro. Each cycle has three steps (1) Denaturation (2) Primer annealing (3) Extension of primer

Refer NCERT BOOK Class 12th Page No.202 (Fig.11.6)

#8. A gene whose expression helps to identify transformed cell is known as

Selectable marker

Vector

Plasmid

Structural gene

In recombinant DNA technology, selectable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

#9. DNA fragments are

Positively charged

Negatively charged

Neutral

Either positively or negatively charged depending on their size

DNA fragments are negatively charged because of phosphate group.

#10. The DNA fragments separated on an agarose gel can be visualised after staining with

Bromophenol blue

Acetocarmine

Aniline blue

Ethidium bromide

Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light. (Refer Class 12th Page-198 Fig.11.3 )

#11. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

The larger the fragment size, the farther it moves

The smaller the fragment size, the farther it moves

Positively charged fragments move to farther end

Negatively charged fragments do not move

During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.

#12. Stirred-tank bioreactors have been designed for

Purification of product

Addition of preservatives to the product

Availability of oxygen throughout the process

Ensuring anaerobic conditions in the culture vessel

#13. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Eco RI

Taq polymerase

Polymerase III

Ligase

In DNA recombinant technology, linking of foreign DNA and plasmid is made possible by DNA ligase which is also called “molecular glue”.

#14. Which of the following is not a component of downstream processing?

Separation

Purification

Preservation

Expression

#15. Which of the following restriction enzymes produces blunt ends?

Sal I

Eco RV

Xho I

Hind III

It cuts the DNA in the centre of restriction site

#16. Which of the following is not a feature of the plasmids?

Single-stranded

Independent replication

Circular structure

Transferable

Plasmid is extrachromosomal, double stranded circular DNA.

#17. The taq polymerase enzyme is obtained from

Pseudomonas putida

Thermus aquaticus

Thiobacillus ferroxidans

Bacillus subtilis

Taq polymerase is thermostable DNA polymerase obtained from Thermus aquaticus.

#18. Which of the following is a restriction endonuclease?

RNase

Hind II

Protease

DNase I

#19. The DNA molecule to which the gene of interest is integrated for cloning is called

Carrier

Transformer

Vector

Template

#20. The cutting of DNA at specific locations became possible with the discovery of

Ligases

Restriction enzymes

Probes

Selectable markers

Restriction enzymes is called molecular scissors or knife.

#21. Which vector can clone only a small fragment of DNA?

Bacterial artificial chromosome

Yeast artificial chromosome

Plasmid

Cosmid

#22. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

Polymerase chain reaction

Electrophoresis

Restriction mapping

Centrifugation

#23. Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

Plant cells - Cellulase

Algae - Methylase

Fungi - Chitinase

Bacteria - Lysozyme

#24. Which one is a true statement regarding DNA polymerase used in PCR?

It is isolated from a virus

It remains active at high temperature

It is used to ligate introduced DNA in recipient cells

It serves as a selectable marker

#25. For transfomation, micro-particles coated with DNA to be bombarded with gene gun are made up of

Silicon or Platinum

Gold or Tungsten

Silver or Platinum

Platinum or Zinc

#26. In genetic engineering, the antibiotics are used

To keep the cultures free of infection

As selectable markers

To select healthy vectors

As sequences from where replication starts

In some case ( To keep the cultures free of infection ) this option may be correct

#27. There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

coli

colon

coelom

coenzyme

#28. Which one of the following techniques made it possible to genetically engineer living organisms?

Heavier isotope labelling

Hybridization

Recombinant DNA techniques

X-ray diffraction

Finish

Results

Cogratulations !

You have passed the Quiz !

Please Review Your answer with Correct Answers and Explanations.

Scroll Down for more Quizzes

Oops !

You have not passed the Quiz !