Details of Bitechnology : Principles and Processes Biology Quiz for NEET
| No. of Questions in Quiz | 28 with Explanation |
| Time for each Questions | 60 Seconds |
| Attempts Allowed | Unlimited |
| Available | Always |
| Pass Percentage | 70 % |
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#1. A selectable marker is used to
#2. An enzyme catalysing the removal of nucleotides from ends of DNA is
Restriction enzymes belong to a larger class of enzymes called Nucleases.
Nucleases are of two different kinds :Exonucleases which remove nucleotides from the end of a DNA molecule, Endonucleases, which break internal phosphodiester bonds at palindromic sites that are highly specific
DNA ligase helps in joining DNA fragments
#3. The two antibiotic resistance genes on vector pBR322 are for
#4. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
#5. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
During the isolation of desired gene, chilled ethanol is used for the precipitation of DNA.
#6. Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbone.
#7. The correct order of steps in Polymerase Chain Reaction (PCR) is
This technique is used for making multiple copies of gene of interest in vitro. Each cycle has three steps (1) Denaturation (2) Primer annealing (3) Extension of primer
Refer NCERT BOOK Class 12th Page No.202 (Fig.11.6)
#8. A gene whose expression helps to identify transformed cell is known as
In recombinant DNA technology, selectable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
#9. DNA fragments are
DNA fragments are negatively charged because of phosphate group.
#10. The DNA fragments separated on an agarose gel can be visualised after staining with
Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light. (Refer Class 12th Page-198 Fig.11.3 )
#11. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.
#12. Stirred-tank bioreactors have been designed for
#13. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
In DNA recombinant technology, linking of foreign DNA and plasmid is made possible by DNA ligase which is also called “molecular glue”.
#14. Which of the following is not a component of downstream processing?
#15. Which of the following restriction enzymes produces blunt ends?
It cuts the DNA in the centre of restriction site
#16. Which of the following is not a feature of the plasmids?
Plasmid is extrachromosomal, double stranded circular DNA.
#17. The taq polymerase enzyme is obtained from
Taq polymerase is thermostable DNA polymerase obtained from Thermus aquaticus.
#18. Which of the following is a restriction endonuclease?
#19. The DNA molecule to which the gene of interest is integrated for cloning is called
#20. The cutting of DNA at specific locations became possible with the discovery of
Restriction enzymes is called molecular scissors or knife.
#21. Which vector can clone only a small fragment of DNA?
#22. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
#23. Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
#24. Which one is a true statement regarding DNA polymerase used in PCR?
#25. For transfomation, micro-particles coated with DNA to be bombarded with gene gun are made up of
#26. In genetic engineering, the antibiotics are used
In some case ( To keep the cultures free of infection ) this option may be correct
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Awesome quiz
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Your questions are FYQ – FUTURE YEAR QUESTIONS . They are Great. Worth sharing to Friends. Waiting for chemistry and physics sections. THANK YOU
thanks please share with friends
I obtained 108 marks but it shows 107 why
some technical issue, may be
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