Bitechnology : Principles and Processes Biology Quiz for NEET

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Details of Bitechnology : Principles and Processes Biology Quiz for NEET
No. of Questions in Quiz 28 with Explanation
Time for each Questions 60 Seconds
Attempts Allowed Unlimited
Available Always
Pass Percentage 70 %

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#1. A selectable marker is used to

Selectable markers help in identification and elimination of non-transformants whilst permitting selective growth of transformants.

#2. An enzyme catalysing the removal of nucleotides from ends of DNA is

Restriction enzymes belong to a larger class of enzymes called Nucleases.

Nucleases are of two different kinds :Exonucleases which remove nucleotides from the end of a DNA molecule, Endonucleases, which break internal phosphodiester bonds at palindromic sites that are highly specific

DNA ligase helps in joining DNA fragments

#3. The two antibiotic resistance genes on vector pBR322 are for

#4. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

#5. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with

During the isolation of desired gene, chilled ethanol is used for the precipitation of DNA.

#6. Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbone.

#7. The correct order of steps in Polymerase Chain Reaction (PCR) is

This technique is used for making multiple copies of gene of interest in vitro. Each cycle has three steps (1) Denaturation (2) Primer annealing (3) Extension of primer

Refer NCERT BOOK Class 12th Page No.202 (Fig.11.6)

#8. A gene whose expression helps to identify transformed cell is known as

In recombinant DNA technology, selectable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.

#9. DNA fragments are

DNA fragments are negatively charged because of phosphate group.

#10. The DNA fragments separated on an agarose gel can be visualised after staining with

Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light. (Refer Class 12th Page-198 Fig.11.3 )

#11. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

During gel electrophoresis, DNA fragments separate according to their size through sieving effect provided by agarose gel.

#12. Stirred-tank bioreactors have been designed for

#13. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

In DNA recombinant technology, linking of foreign DNA and plasmid is made possible by DNA ligase which is also called “molecular glue”.

#14. Which of the following is not a component of downstream processing?

#15. Which of the following restriction enzymes produces blunt ends?

It cuts the DNA in the centre of restriction site

#16. Which of the following is not a feature of the plasmids?

Plasmid is extrachromosomal, double stranded circular DNA.

#17. The taq polymerase enzyme is obtained from

Taq polymerase is thermostable DNA polymerase obtained from Thermus aquaticus.

#18. Which of the following is a restriction endonuclease?

#19. The DNA molecule to which the gene of interest is integrated for cloning is called

#20. The cutting of DNA at specific locations became possible with the discovery of

Restriction enzymes is called molecular scissors or knife.

#21. Which vector can clone only a small fragment of DNA?

#22. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

#23. Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

#24. Which one is a true statement regarding DNA polymerase used in PCR?

#25. For transfomation, micro-particles coated with DNA to be bombarded with gene gun are made up of

#26. In genetic engineering, the antibiotics are used

In some case ( To keep the cultures free of infection ) this option may be correct

#27. There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

#28. Which one of the following techniques made it possible to genetically engineer living organisms?




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46 thoughts on “Bitechnology : Principles and Processes Biology Quiz for NEET”

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  1. The quizzes are amazing and good quality questions.. they consist of pyqs too.. thankyou so much❤️
    The explanation below the wrong answers helps so much
    They are amazing!


  3. Unit wise and full syallabus bhi daaliye na bht mazza aata h aise quiz krne m wrna bioo jyda theory pdhta hu toh bore hojata hu

  4. I think lots of questions in your quizes are from previous year paper, It will be better if you provide different but yes only from ncert

  5. Very good initiative….. time saving tip everyone should attempt…. plz start for full syllabus if convenient for u…

  6. Your questions are FYQ – FUTURE YEAR QUESTIONS . They are Great. Worth sharing to Friends. Waiting for chemistry and physics sections. THANK YOU


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